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    Welcome to AMBIO!

    AMBIO offers both infrastructure and expertise to perform advanced microscopy projects.

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    New User Registration

    AMBIO services are available to Charité workgroups, associated academic institutions, and external institutions.

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    AMBIO Systems

    The microscopy imaging systems at AMBIO were chosen to be highly complementary and cover many imaging modes.

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    Research at AMBIO

    The Axonal Membrane Protein PRG2 Inhibits PTEN and Directs Growth to Branches

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    Research at AMBIO

    Lipid-mediated PX-BAR domain recruitment couples local membrane constriction to endocytic vesicle fission

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    Research at AMBIO

    Novel organic dyes for multicolor localization‐based super‐resolution microscopy

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    Research at AMBIO

    Diffusional spread and confinement of newly exocytosed synaptic vesicle proteins

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    Research at AMBIO

    Multicolor Caged dSTORM Resolves the Ultrastructure of Synaptic Vesicles in the Brain

Advanced Medical BioImaging Core Facility – Nikon Center of Excellence for Microscopy at the Charité

The Advanced Medical BioImaging Core Facility (AMBIO) at the Charité-Universitätsmedizin has been established to provide both the infrastructure and expertise necessary for researchers with diverse backgrounds to efficiently perform advanced fluorescence microscopy imaging projects on the molecular, cellular and tissue level.

In partnership with Nikon Instruments Germany the Charité established the initial instrumental core of AMBIO as the first 'Nikon Center of Excellence' in Berlin.

AMBIO services include individual user training and assistance, and if necessary supervised image acquisition and guidance for data analysis.

AMBIO currently offers the following imaging modes:   

  • Widefield Imaging
  • Scanning & Spinning Disk Confocal Imaging
  • TIRF Imaging
  • Multiphoton Confocal Imaging
  • Super-Resolution Imaging (STORM/PALM, SIM)

All AMBIO systems are equipped to allow multiplexed live-cell imaging projects, including time-lapse, multi-color, multi-position, large image scan and multi-well automation projects. The large range of available objectives offers imaging of diverse sizes of samples over scales ranging from the nanoscale (single molecules, sub-cellular structures) to the millimeter scale (tissues, small organs).