AMBIO Systems

Systems at AMBIO

The complementary imaging systems at AMBIO are able to image a wide variety of samples (i.e. tissue culture cells, tissue sections, small organs) over scales ranging from the mesoscale (>1 mm) to the nanoscale (<20 nm).

All AMBIO systems are equipped for multiplexed 3D live-cell experiments:

Multi-color, multi-position, time series, large image scan, automation
Environmental stage control (temperature, humidity + CO₂, carbogen)
Perfusion
Autofocus/ focus drift correction (PFS, Nikon systems)
Fully motorized high precision stage for accurate repositioning (3D x/y/z control)

Link: Open IRIS Booking Schedule Book AMBIO systems here.

AMBIO Systems Applications Guide

Click for an overview about possible applications in AMBIO.

Current imaging systems

ZEISS Lattice Lightsheet 7

Long-term ultra-fast 3D subcellular imaging of living cells in standard sample carriers with minimal phototoxicity and bleaching. Available imaging lasers: 488 nm, 561 nm, and 640 nm. Additionally, the system is equipped with a laser scanning-based photo activation/manipulation system.

Nikon Widefield Ti2

Inverted widefield microscope for EPI fluorescence and bright-field modes equipped with 6 LED light sources, 4-megapixel sCMOS camera and 16-megapixel color CMOS camera for histology slides. Specials: fast LED laser switching/ tuning, and fast emission filter wheel for clean multi-color imaging.

Nikon Scanning Confocal A1Rsi+

Inverted confocal laser scanning microscope equipped with 4 lasers, galvano/ resonant scanner, highly sensitive Hybrid GaAsP PMTs, and a 32 PMT array for spectral detection/ spectral unmixing. Detection of transmitted light (DIC, phase) and FRAP/ PA (photo activation/ conversion) available.

Nikon Spinning Disk Confocal CSU-X

Inverted spinning disk confocal for fast live-cell imaging equipped with 4 lasers and 2 cameras for ultra-fast dual color imaging. Specials: FRAP/ PA (photo activation/ conversion), fast emission filter wheel for clean multi-color imaging, and ultra-fast laser switching/ tuning (triggered acquisition).

Nikon CSU-W1 SoRa

Inverted spinning disk confocal for fast live-cell imaging equipped with 7 lasers and 2 cameras for ultra-fast dual color imaging. Specials: large FOV, enhanced signal-to-noise ratio, enhanced resolution (SoRa-mode), FRAP/ PA (photo activation/ conversion), and fast emission filter wheel for clean multi-color imaging.

Nikon Upright Spinning Disk Confocal S2

Upright spinning disk confocal for fast live-cell imaging equipped with 4 lasers. Specials: fast emission filter wheel for clean multi-color imaging and ultra-fast laser switching/ tuning (triggered acquisition).

Nikon A1R+ Multiphoton Confocal

Upright multiphoton confocal for deep tissue/ organ/ intravital imaging equipped with galvano/ resonant scanner and dual IR laser (1040 nm and 680-1300 nm tunable).

Nikon Ti-E TIRF/STORM

Inverted widefield micrsocope for imaging dynamic membrane processes in live cells (TIRF) and super-resolution/ single molecule localization (dSTORM) equipped with 4 lasers and 1.44-megapixel sCMOS camera (Photometrics Prime 95B). Specials: FRAP/ PA (photo activation/ conversion), ultra-fast laser switching/ tuning (triggered acquisition), high-speed emission filter wheel for clean multi-color imaging, and emission splitter for simultaneous 2- or 3-color imaging.

Image analysis workstations

Analysis Workstation 1 (64GB)

Hardware

CPU: Intel Xeon 3.40 GHz, 64GB RAM
Graphic: NVIDIA Quadro K4000, GPU Memory 3GB

Software

Windows 7 64-bit System
MS Office 2013

ARIVIS 4D Vision (2.12.5) – upon request
NIKON NIS-Elements AR Analysis – upon request
Fiji (1.51n)
MATLAB – upon request
RStudio (3.5.3)
Inkscape (0.91)
Gimp (2.8.22)

Analysis Workstation 2 (32GB)