Advanced Medical BioImaging Core Facility 

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    Campus Charité MitteCharitéplatz 1 (local address: Virchowweg 6)
    10117 Berlin

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Nikon Scanning Confocal A1Rsi+

Charité Campus Mitte, CCO Rm. 01-306, Virchowweg 6, 10117 Berlin, Germany

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Specifications

Imaging modes

  • Scanning confocal (DAPI, GFP, mCherry, Cy5)
  • Transmitted light detector (DIC, phase)
  • FRAP/ PA (photo activation/ conversion)
  • Live-cell incubator (temperature, CO2, humidity)
  • Spectral scans/ spectral unmixing

Lasers and max. intensities out of 60x oil objective

  • 405 nm: 0.44 mW
  • 488 nm: 0.8 mW 
  • 561 nm: 1.66 mW 
  • 647 nm: 1.7 mW

Scanner

  • Point scanner (galvo)
  • Resonant scanner (30 fps in 512x512 pixels, up to 420 fps in 512x32 pixels)
  • Pinhole: hexagonal, tunable

Detectors

  • 2x Hybrid GaAsP PMT (high sensitivity, GFP, mCherry)
  • 2x PMT (Dapi, Cy5)
  • Transmission light detector (DIC, phase)
  • Spectral detector (optical diffraction grid and 32-channel multianodes)

Special

  • Fast switch between galvo and resonant scanners
  • Spectral scanner (unmixing)
  • Piezo z-stage insert for ultra-fast and precise focussing (Mad City Labs)