Advanced Medical BioImaging Core Facility 

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    Campus Charité MitteCharitéplatz 1 (local address: Virchowweg 6)
    10117 Berlin

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Nikon Spinning Disk Confocal CSU-X

Charité Campus Mitte, CCO Rm. 01-306, Virchowweg 6, 10117 Berlin, Germany

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Specifications

Imaging modes

  • Spinning disk confocal (DAPI, GFP, mCherry, Cy5)
  • FRAP/ PA (photo activation/ conversion)
  • Live-cell incubator (temperature, CO2, humidity)

Lasers and max. intensities out of 60x oil objective

  • 405 nm: 3.54 mW
  • 488 nm: 3.85 mW 
  • 561 nm: 4.11 mW 
  • 647 nm: 4.68 mW

Detectors

  • 2 EMCCD cameras (iXon3 DU-888 Ultra, 1024x1024 pixels, 13 μm pixel size, 26 fps full frame, usable area ~512x512 pixels, 93 fps)
  • Current dual color dichroic for simultaneous GFP & mCherry detection

Special

  • Andor Revolution SD System (CSU-X)
  • Uniform spinning disk illumination
  • 2 cameras for ultra-fast dual color imaging
  • Fast emission filter wheel for clean multi-color imaging of DAPI, GFP, mCherry, and Cy5
  • Ultra-fast laser switching/ tuning (triggered acquisition)
  • Piezo z-stage insert for ultra-fast and precise focussing (Mad City Labs)