Nikon Ti-E TIRF/STORM

Imaging modes

• Widefield EPI fluorescence (DAPI, GFP, mCherry, Cy5)
• Phase (10x, 20x)
• DIC (20x, 40x, 100x)
• TIRF (adhesive cell membrane), motorized TIRF angle 
• dSTORM (super-resolution, single molecule localization) 
• FRAP/ PA (photo activation/ conversion)
• Live-cell incubator (temperature, CO₂, humidity)

Lasers and max. intensities out of 100x oil objective

• 405 nm: 3.31 mW
• 488 nm: 53.5 mW 
• 561 nm: 37.4 mW 
• 647 nm: 80.7 mW

Detectors

• Camera 1 (left port): EMCCD, Andor iXon Ultra X3 987, 512x512 pixels, 16 µm pixel size, 56 fps full frame, QE max. 90%
• Camera 2 (right port): sCMOS, Photometrics Prime 95B (95% QE, 1200x1200 pixels, 11 µm pixel size, 41 fps full frame, 82 fps@12-bit)
• Camera 3 (left front port): CMOS, The Imaging Source DMK 23UX249 (1920x1200 pixels, 5.86 µm pixel size, 30 fps full frame)

Special

• 2x beam condenser for small FOV (dSTORM)
• Ultra-fast laser switching/ tuning (triggered acquisition)
• Piezo z-stage insert for ultra-fast and precise focussing (Mad City Labs)