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    Campus Charité MitteCharitéplatz 1 (local address: Virchowweg 6)
    10117 Berlin

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Charité Campus Mitte, CCO Rm. 01-306, Virchowweg 6, 10117 Berlin, Germany

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Imaging modes

  • Widefield EPI fluorescence (DAPI, GFP, mCherry, Cy5)
  • Phase (10x, 20x)
  • DIC (20x, 40x, 100x)
  • TIRF (adhesive cell membrane), motorized TIRF angle 
  • dSTORM (super-resolution, single molecule localization) 
  • FRAP/ PA (photo activation/ conversion)
  • Live-cell incubator (temperature, CO2, humidity)

Lasers and max. intensities out of 100x oil objective

  • 405 nm: 3.31 mW
  • 488 nm: 53.5 mW 
  • 561 nm: 37.4 mW 
  • 647 nm: 80.7 mW


  • Camera 1 (left port): EMCCD, Andor iXon Ultra X3 987, 512x512 pixels, 16 µm pixel size, 56 fps full frame, QE max. 90%
  • Camera 2 (right port): sCMOS, Photometrics Prime 95B (95% QE, 1200x1200 pixels, 11 µm pixel size, 41 fps full frame, 82 fps@12-bit)
  • Camera 3 (left front port): CMOS, The Imaging Source DMK 23UX249 (1920x1200 pixels, 5.86 µm pixel size, 30 fps full frame)


  • 2x beam condenser for small FOV (dSTORM)
  • Ultra-fast laser switching/ tuning (triggered acquisition)
  • Piezo z-stage insert for ultra-fast and precise focussing (Mad City Labs)