Nikon Widefield Ti2
Charité Campus Mitte, CCO Rm. 01-306, Virchowweg 6, 10117 Berlin, Germany
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Specifications
Imaging Modes
- Widefield EPI fluorescence (DAPI, CFP, GFP, YFP, mCherry, Cy5)
- Bright-field (transmitted light) for histological slides (color stains)
- Automated phase for 10x, 20x objectives
- Live-cell incubator (temperature, CO2, humidity)
- Multiplexing (multi position, large image scan, automation)
- Water immersion objectives for high-resolution on water based samples
- 3D Deconvolution
LED Illumination (Lumencore, SpectraX) and max. intensities out of 40x objective
- 395/25 nm: 66.5 mW
- 438/29 nm: 109 mW
- 475/28 nm: 57 mW
- 511/16 nm: 12.3 mW
- 555/28 nm: 145 mW
- 635/22 nm: 38.9 mW
Detectors
- sCMOS, PCO.edge camera (>80% QE, 2048x2048 pixels, 6.5 µm pixel size, 53 fps full frame, up to 500 fps in small area) > Fluorescent samples
- Nikon DS-Ri2 color CMOS camera (4908x3264 pixels, 6 fps full frame, 45 fps in 1636x1088 pixels) > Stained samples (histology)
Special
- Fast LED laser switching/ tuning (triggered acquisition)
- Fast emission filter wheel for clean multi-color imaging of DAPI, CFP, GFP, YFP, mCherry, and Cy5
- Automatic water immersion dispenser (WID) for water immersion objectives
- 3D Deconvolution
- NIS-Elements AR (Advanced Research) software including modules for:
- JOBS (automation)
- Deconvolution
- NIS.ai (Artificial Intelligence): denoise, clarify, enhance, segment, convert
