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    Campus Charité MitteCharitéplatz 1 (local address: Virchowweg 6)
    10117 Berlin

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Nikon Widefield Ti2

Charité Campus Mitte, CCO Rm. 01-306, Virchowweg 6, 10117 Berlin, Germany

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Imaging Modes

  • Widefield EPI fluorescence (DAPI, CFP, GFP, YFP, mCherry, Cy5)
  • Bright-field (transmitted light) for histological slides (color stains)
  • Automated phase for 10x, 20x objectives
  • Live-cell incubator (temperature, CO2, humidity)
  • Multiplexing (multi position, large image scan, automation)
  • Water immersion objectives for high-resolution on water based samples
  • 3D Deconvolution
  • Interference Reflection Microscopy (IRM)

LED Illumination (Lumencore, SpectraX) and max. intensities out of 40x objective

  • 395/25 nm: 66.5 mW
  • 438/29 nm: 109 mW
  • 475/28 nm: 57 mW
  • 511/16 nm: 12.3 mW
  • 555/28 nm: 145 mW
  • 635/22 nm: 38.9 mW


  • sCMOS, PCO.edge camera (>80% QE, 2048x2048 pixels, 6.5 µm pixel size, 53 fps full frame, up to 500 fps in small area) > Fluorescent samples
  • Nikon DS-Ri2 color CMOS camera (4908x3264 pixels, 6 fps full frame, 45 fps in 1636x1088 pixels) > Stained samples (histology)


  • Fast LED laser switching/ tuning (triggered acquisition)
  • Fast emission filter wheel for clean multi-color imaging of DAPI, CFP, GFP, YFP, mCherry, and Cy5
  • Automatic water immersion dispenser (WID) for water immersion objectives
  • 3D Deconvolution
  • NIS-Elements AR (Advanced Research) software including modules for:
    • JOBS (automation)
    • Deconvolution
    • (Artificial Intelligence): denoise, clarify, enhance, segment, convert