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3D Live Cell Imaging

Most AMBIO’s systems are configured to perform high-resolution multiplexed imaging experiments to reveal dynamics of subcellular structures over time (3D, live sample, multicolor).

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Spinning Disk Confocal

The Nikon CSU-X is so far the most suited system to acquire 3D high-resolution images from live cells. AMBIO users have performed over 100 projects within the last years on this system. Some of the projects were recently published:

Nanoscale Coupling of Endocytic Pit Growth and Stability. Lehmann M, Lukonin I, Noé F, Schmoranzer J, Clementi C, Loerke D, Haucke V. Sci Adv. 2019

The Axonal Membrane Protein PRG2 Inhibits PTEN and Directs Growth to Branches. Brosig A, Fuchs J, Ipek F, Kroon C, Schrötter S, Vadhvani M, Polyzou A, Ledderose J, van Diepen M, Holzhütter HG, Trimbuch T, Gimber N, Schmoranzer J, Lieberam I, Rosenmund C, Spahn C, Scheerer P, Szczepek M, Leondaritis G, Eickholt BJ. Cell Rep. 2019

A SEPT1-based Scaffold Is Required for Golgi Integrity and Function. Song K, Gras C, Capin G, Gimber N, Lehmann M, Mohd S, Puchkov D, Rödiger M, Wilhelmi I, Daumke O, Schmoranzer J, Schürmann A, Krauss M. J Cell Sci. 2019

Nesprin-1α-Dependent Microtubule Nucleation From the Nuclear Envelope via Akap450 Is Necessary for Nuclear Positioning in Muscle Cells. Gimpel P, Lee YL, Sobota RM, Calvi A, Koullourou V, Patel R, Mamchaoui K, Nédélec F, Shackleton S, Schmoranzer J, Burke B, Cadot B, Gomes ER. Curr Biol. 201

Lattice Light Sheet

We are proud to announce that we recently received a grant to acquire a novel Lattice Light Sheet Microscope System (LLS) within the DFG grant scheme ‘Neuartige Lichtmikroskopie/Novel Light Microscopy’

With this system AMBIO significantly enhances its main focus on high resolution live cell imaging. The LLS will allow AMBIO users to perform ultra-fast 3D subcellular imaging with minimal phototoxicity. The system is planned to be installed in Summer 2020.