Super-Resolution within SFB 958/Z02
Within AMBIO and the SFB 958/Z02 project we develop and apply advanced imaging methods, such as 3D multicolor super-resolution, to resolve nanoscale structures and dynamics in diverse cellular systems.
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Our projects include high- and super-resolution imaging as well as customized 3D image analysis. Currently, we offer several super-resolution methods that all achieve an improved spatial resolution beyond the Abbe limit (~ 200 nm) for light microscopy. These methods include Structured Illumination Microscopy (SIM) and Single Molecule Localization Microscopy (SMLM). Stimulated Emission Depletion (STED) is offered by other groups within Berlin (Sigrist lab, FMP Cellular Imaging core facility, FU Berlin BioSupraMol core facility).
Structured Illumination Microscopy (SIM)
SIM is based on a diffraction limited structured illumination pattern and can achieve factor 2 higher resolution in all dimensions through computational 3-dimensional reconstruction. Main advantage are the relatively low illumination densities compared to SMLM and STED while using regular probes and being intrinsically 3-dimensional.
Single Molecule Localization Microscopy (SMLM)
SMLM uses the accuracy of single molecule localization to achieve a spatial resolution beyond the up to about 10 nm. Currently, we optimize and implement diverse variants of SMLM, including STORM, PALM and DNA-PAINT.
Stimulated Emission Depletion (STED)
STED is based on local quenching of fluorescence via a doughnut shaped beam that effectively leads to a much small emission point spread function. STED is a laser scanning based method that can achieve a spatial resolution of a few tens of nm, depending on the illumination density.